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In-Gel Detection of DNA: Application to Study of Viral DNA Metabolism by Use of Pulsed-Field Agarose Gel Electrophoresis
Time of Update: 2020-12-28
The introduction of pulsed-field agarose gel electrophoresis (PFGE) has expanded the list of particles separable by use of gel electrophoresis to include: (1) linearDNA s as long as 3–6 Mbp, (2) DNA- protein complexes and circular DNAs that become arrested during invariant field agarose gel electrophoresis; and (3) micron-sized spheres that also become arrested during invariant field agarose gel electrophoresis (reviewed in refs.
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Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels
Time of Update: 2020-12-03
The constraint of the capillary format also acts to prevent convection, whereas in the slab-gel format the sieving matrix plays this role.
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The horizontal agarose gel electrophoresis method detects DNA
Time of Update: 2020-11-03
Experimental Principles Closed ring-shaped, mitochondrial and open-ringed granulate DNA, due to their different configuration, show different migration rates on agarose gel electrophoresis of abrominated ethyl ingots, so that observations under ultraviolet light can distinguish between closed ring-like proton DNA (cccDNA), mitochondrial DNA (L-DNA) and open-loop granulated DNA (ocDNA).
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Agarose gel electrophoresis identifies DNA
Time of Update: 2020-10-31
The electrophoresis method of horizontal plate agarose gel electrophoresis with constant electrophoresis strength and electrophoresis direction can be used to separate DNA fragments at a length of 50-20,000 bp.
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Dna fragments of agarose gel electrophoresis.
Time of Update: 2020-10-30
DNA molecules of different sizes, shapes, and images have different migration rates under the same electrophoresis conditions (e.g. gel concentration, current, voltage, buffer, etc.), so they can be separated by electrophoresis.
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DNA's agarose gel electrophoresis.
Time of Update: 2020-10-25
EDTA buffer: 10.78g Tris, 5.500g boric acid, 0.930g EDTA-Na2 dissolved in ionic water, fixed to 100ml, diluted 10 times in time; EB solution: ethyl bromide ingots (10mg/ml) (diluted 10 times over time); Sample buffer: 50% glycelin plus 0.25% bromophenol blue; 3, experimental steps (i) preparation of agarose gel: 1.
