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Analysis of Cytochrome P450 Polymorphisms
Time of Update: 2020-12-25
This chapter describes the detection of common polymorphisms in cytochrome P450 genes using both genotyping and phenotypmg approaches.
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Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity
Time of Update: 2020-12-21
Combinatorial fluorescence energy transfer (CFET) tags, constructed by exploiting fluorescence energy transfer and combinatorial synthesis to generate a large number of unique fluorescence emission signatures from a limited number of fluorophores, allow multiple biological targets to be identified simultaneously.
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Single-Nucleotide Polymorphisms: Technology and Applications
Time of Update: 2020-12-18
The completion of the human genome project and the application of highthroughput technologies for polymorphism detection to the investigation of complex disease genetics has created unprecedented opportunities for understanding the pathogenic basis of common human diseases ( 1 , 16 ).
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Invader Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation
Time of Update: 2020-12-13
The Invader� assay (Third Wave Technologies) is a homogeneous, isothermalDNA probe-based method for sensitive detection of nucleic acid sequences.
Detection is achieved through target-specific signal amplification instead of target amplification.
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Single-Strand Conformation Polymorphism (SSCP) Analysis
Time of Update: 2020-12-13
Although we have recently seen a considerable improvement in our ability to detectDNA alterations, costly mutation analysis using nucleotide sequencing has driven a search for less expensive scanning methods.
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Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing
Time of Update: 2020-12-12
We have expanded the utility of this technique to analyze easily the alternative splicing of pre-mRNAs containingmultiple mutually exclusive exons of the same size.
The ease and the quantitative nature of this technique should be very useful.
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Random Amplified Polymorphic DNA (RAPDs)
Time of Update: 2020-11-30
Because polymorphisms are noted by the presence or absence of amplification products from a single allele, the random amplified polymorphic DNA (RAPD) technique tends to provide only dominant markers.
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Restriction Fragment Length Polymorphism
Time of Update: 2020-11-27
Some of these polymorphisms that occur with a high frequency within the population can be a useful tool for gene tracking for a given disease.
2. Allele specific oligonucleotide hybridization (ASO-H) or similar techniques.
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Restriction Fragment-Length Polymorphisms
Time of Update: 2020-11-24
Knowledge of eukaryotic-gene structure has grown exponentially over the past two decades, mainly owing to the mtroduction of new molecular techniques These have enabled the manipulation and analysis
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Restriction Fragment-Length Polymorphisms
Time of Update: 2020-11-23
Knowledge of eukaryotic-gene structure has grown exponentially over the past two decades, mainly owing to the mtroductionof new molecular techniques These have enabled the manipulation and analysis of
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Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-Stress Response in Montepulciano and Sangiovese Grape Cultivars
Time of Update: 2020-11-21
Methylation-sensitive amplified polymorphism (MSAP) is a technique developed for assessing the extent and pattern of cytosine methylation and has been applied to genomes of several species (Arabidops
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Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism
Time of Update: 2020-11-20
RFLPs are typically detected as DNA fragments of different lengths after digestion with various restriction endonucleases.
Since DNA can be digested with methylation sensitive enzymes, the assay can also be used to analyze a methylation pattern of a particular locus.
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Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes
Time of Update: 2020-11-20
One of them is a modification of the Amplified Fragment Length Polymorphism (AFLP) technique that has been used to study methylation of anonymous CCGG sequences in different fungi, plant and animal species.
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ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism
Time of Update: 2020-11-18
These marker systems differ in their advantages and disadvantages, so it is imperative to keep in mind all the pros and cons of the technique while selecting one for the problem to be addressed.
We have outlined here the whole methodology of this technique with an example ofTribulus terrestris as case study.
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Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies
Time of Update: 2020-11-07
Amplified fragment length polymorphism (AFLP) is aPCR -based technique that uses selective amplification of a subset of digestedDNA fragments to generate and compare unique fingerprints for genomes of interest.
Variations of standard AFLP methodology have been also developed for targeting additional levels of diversity, such as transcriptomic variation and DNA methylation polymorphism.