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Preparation of commonly used reagents in molecular biology.
Time of Update: 2020-10-24
1. LB (Luria-Bertani) culture liquid, plate preparation preparation per liter of LB culture, should be added in 950 ml of deionized water: bacteria culture with yeast extract (bacto-yeastextract) 5g bacteria culture with totalin (bac-tryptone) 10g NaCl10g shakes the container until the solute dissolves completely, adjusts the pH to 7.0 with 5mol/LNaOH (about 0.2ml), adds deionized water to a total volume of 1L, and steams sterilise 20min at 151bf/in2 (1.034×105Pa) pressure.
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The preparation of experimental biological reagents is commonly used in laboratories.
Time of Update: 2020-10-22
No Ca2 plus, Mg2 plus Hanks liquid NaCl 4.5g KCl 0.2g NaHCO3 0.175g Na2HPO4.12H2O 0.076g KH2PO4 0.03g glucose 0.5g 0.4% phenol red 2.5ml dissolves or adds the above ingredients to the double steamed water in turn, Finally add double steamed water to 500ml, Adjust the pH to 7.4, 4 degrees C refrigerator to save the spare at 5.6% NaHCO3.
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He dyeing reagent preparation is commonly used.
Time of Update: 2020-10-21
Dilute 1:1 to 2 times with 95% alcohol before use and add 1 to 2% ice acetic acid.
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Reagent preparation methods are commonly used.
Time of Update: 2020-10-21
7. Dinitrobenzene test liquid take 2,4-dinitrobenzene 1.5g, sulfuric acid solution (1→2) 20ml, dissolved, water into 100ml, filtered, that is.
19.Sodium nitrite ethanol test liquid take 5g of sodium nitrite, plus 60% ethanol to dissolve into 1000ml, that is.
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A small synth of several commonly used brominated reagents.
Time of Update: 2020-10-21
-related topics NBS brominated reagents and brominated reactions as the title, recently in a brominated reaction, niobium-based alpha bit, want to talk to you what commonly used brominated reagents ah, such as liquid bromine, NBS, DBBA, DBBI, and a bibrominated hyinein, said to be cheap, industrial use, laboratory small amount of other words can be NBS.
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Molecular biology is commonly used in reagent configurations.
Time of Update: 2020-10-19
, Molecular Biology the preparation of commonly used storage fluids 1, 30% acrylamide solution (preparation method) 29g acrylamide and 1g N, N'-methylene diacrylamide dissolved in a total volume of 60ml of water.
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The principle of the function of bio-chemical reagents commonly used in molecular experiments is summarized.
Time of Update: 2020-10-19
Phenols, chloroform and isotenol ratio of 25:24:1 (do not have to be pre-provisioned, can be added to a 24:1 chloroform and isosterol before use), while isotrol helps to phase, so that the centrifugal upper water phase, the middle denatured protein phase and the lower organic solvent phase to maintain stability.
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Specificity greater than 99.8%! Roche's new coronary serological testing reagent sedited by FDA for emergency use
Time of Update: 2020-06-06
Roche has begun shipping the new antibody testing reagent storts to laboratories around the world and will increase its production capacity to support up to tens of millions of tests per month to serv
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New RNA interference reagent can be used to treat ocular inflammation
Time of Update: 2017-02-16
Inflammation plays a central role in visual threatening eye diseases such as age-related macular degeneration and diabetic retinopathy More commonly, it causes uveitis or uveitis Uveitis causes rednes
