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Exploring Protein Superstructures and Dynamics in Live Bacterial Cells Using Single-Molecule and Superresolution Imaging
Time of Update: 2020-12-14
Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the optical diffraction limit, and enables superresolution reconstruction of structures beyond the diffraction limit.
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Peptide Detection and Structure Determination in Live Cells Using Confocal Raman Microscopy
Time of Update: 2020-12-14
Therefore, experimental methodsthat can provide structural information on peptides in live cells are useful for exploring peptide structure–activity relationshipsand metabolism directly within the targeted cellular environment.
In this chapter we describe an experimental methodologyfor the detection and structure determination of exogenous peptides within living cells using confocal Raman microscopy (CRM).
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Imaging Phosphoinositide Dynamics in Living Cells
Time of Update: 2020-12-12
To improve our understanding of the important roles played by inositol lipid derivatives in signalling and other cellular processes, it is crucial to measure phosphoinositide concentration changes in individual cells with high spatial and temporal resolution.
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Force and compliance measurements on living cells using atomic force microscopy (AFM)
Time of Update: 2020-12-07
Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion.
In addition, the AFM was used as a microindenter to determine the mechanical properties of cells.
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Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid
Time of Update: 2020-12-06
Reactive-oxygen species and organic free radicals are essential metabolic intermediates and have important regulatory functions.
Quantitative assays of lipid peroxidation in intact cells are essential for evaluating oxidative damage from various sources, and in testing the efficacy of antioxidant interventions.
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Multiphoton Laser-Scanning Microscopy and Spatial Analysis of Dehydroergosterol Distributions on Plasma Membrane of Living Cells
Time of Update: 2020-11-30
Multiphoton laser-scanning microscopy (MPLSM) imaging in combination with advanced image analysis techniques provides unique opportunities to visualize the arrangement of cholesterol in the plasma membrane (PM) of living cells.
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A 3D imaging microscope can observe living cells
Time of Update: 2020-11-28
, June 30 (Xinhua Mao Li) According to local media reports, Israel University of Technology has successfully developed a new type of microscope that can display three-dimensional images of living cells at ultra-high resolution, which is expected to revolutionized biological research.
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Multiplex Imaging of Rho Family GTPase Activities in Living Cells
Time of Update: 2020-11-27
Here, we provide procedures for imaging the Rho GTPase biosensors in both single and multiplex acquisition modes.
The multiplex approach enables the direct visualization of two biosensor readouts from a single living cell.
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Visualizing Intracellular Distribution and Activity of Core2 (1,6)N-Acetylglucosaminyltransferase-I in Living Cells
Time of Update: 2020-11-23
The core2 β(1,6)- N -acetylglucosaminyltransferase-I (C2GnT-I) is expressed by leukocytes and is involved in the synthesis of core2O -glycans that carry sialyl-Lewis x (sLe x ) oligosaccharides.
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Live Cell Imaging of Arabidopsis Root Hairs
Time of Update: 2020-11-20
Root hairs are tubular extensions from the root surface that expand by tip growth.
This highly focused type of cell expansion, combined with position of root hairs on the surface of the root, makes them ideal cells for microscopic observation.
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Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells
Time of Update: 2020-11-17
However, the study of protein complex formation in living plant cells has remained experimentally difficult and time-consuming and requires sophisticated technical equipment.
In this report, we describe a bimolecular fluorescence complementation (BiFC) technique for visualization of protein–protein interactions in plant cells.
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Measurements of Cytosolic Ion Concentrations in Live Cells
Time of Update: 2020-11-12
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes.
We describe procedures for the use of SBFI for the measurement of Na + in live cells.
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Progress was made in the study of the active metabolites and fat-lowering mechanisms of sponge co-living hair cells.
Time of Update: 2020-09-16
Sponge is a porous filter-eating invertebrate, a habitat for a large number of marine microorganisms, and an important part of marine coral reef ecosystem. Sponge's own physical defense is poor, spon
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Scientists use living cells to create machine fish.
Time of Update: 2020-09-15
Kevin Kit Parker, 's project leader and a Harvard bioengineering scientist, said the researchers used gold to make a flexible "skeleton" with fins made of silicone on top and 200,000 mouse heart muscle cells at the top.
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A new 3D genome living cell imaging tool, TALE.
Time of Update: 2020-09-07
the study developed a new 3D genome living cell imaging tool, TALE, and used the system to achieve accurate imaging of telomere shortening and chromatin structure changes associated with aging, such as changes in silk particle composition.