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Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-Stress Response in Montepulciano and Sangiovese Grape Cultivars
Time of Update: 2020-11-21
Methylation-sensitive amplified polymorphism (MSAP) is a technique developed for assessing the extent and pattern of cytosine methylation and has been applied to genomes of several species (Arabidops
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Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism
Time of Update: 2020-11-20
RFLPs are typically detected as DNA fragments of different lengths after digestion with various restriction endonucleases.
Since DNA can be digested with methylation sensitive enzymes, the assay can also be used to analyze a methylation pattern of a particular locus.
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Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes
Time of Update: 2020-11-20
One of them is a modification of the Amplified Fragment Length Polymorphism (AFLP) technique that has been used to study methylation of anonymous CCGG sequences in different fungi, plant and animal species.
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ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism
Time of Update: 2020-11-18
These marker systems differ in their advantages and disadvantages, so it is imperative to keep in mind all the pros and cons of the technique while selecting one for the problem to be addressed.
We have outlined here the whole methodology of this technique with an example ofTribulus terrestris as case study.
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Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies
Time of Update: 2020-11-07
Amplified fragment length polymorphism (AFLP) is aPCR -based technique that uses selective amplification of a subset of digestedDNA fragments to generate and compare unique fingerprints for genomes of interest.
Variations of standard AFLP methodology have been also developed for targeting additional levels of diversity, such as transcriptomic variation and DNA methylation polymorphism.
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Detection of gene polymorphism
Time of Update: 2020-11-03
The principle is that the PCR gene fragment is amplified by using sequence-specific oligonucleotide probe to analyze and identify the amplification fragment by hybridization.
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Polymorphism of DNA structures
Time of Update: 2020-10-31
In the use of potassium or anti-ion, relative humidity of 75%, the DNA molecule's X-ray diffraction map gives the A composition, A-DNA each spiral contains 11 base pairs, and become A-DNA, the large ditch narrows, becomes deeper, the groove becomes wider and shallower.
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A new immune subject of plant-perceived lynx polymorphism whiplash has been revealed.
Time of Update: 2020-09-13
experts say the study is the first to reveal the allegiance variants in soybeans that identify bacteria polymorphic whiplash, and proves that the identification of this polymorphic whiplash protein can enhance plant disease resistance and provide a new strategy for improving crop resistance to bruising bacteria.
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Effects of polymorphism of enteritis-sensitive genes on intestinal bacteria and Crohn's disease.
Time of Update: 2020-09-11
the exploration of the complex mechanism of symbient-mutual benefit" will be of great significance to the study of the pathogenesis of diseases caused by abnormal intestinal bacteripora, as hosts and intestinal bacteri in the long-term evolutionary process interact to regulate the environmental stability of the intestines.
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Single base polymorphism points (SNPs) on apple chromosomes
Time of Update: 2020-09-10
Under the guidance of researcher Han Yupeng, Dr. Ma Baiquan of the Fruit Tree Molecular Breeding Group of Wuhan Botanical Garden of the Chinese Academy of Sciences simplified genome sequencing of 30 apple species resources and obtained 39,635 single base polymorphism points (SNPs) evenly distributed on apple chromosomes.
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Single nucleotide polymorphism analysis identifies a new set of tumor suppressor genes.
Time of Update: 2020-08-21
problem is that tumors often contain different proportions of healthy and cancerous cells, making it difficult to determine whether one or two tumor suppressor genes are missing from cancer cells.
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Molecular nucleation mechanism and control strategy of crystal polymorphic selection.
Time of Update: 2020-08-10
S. Van Driesche of the University of The Alps in Grenoble , among others, entitled "Molecular nucleation mechanisms and control sstrategies for crystal polymorph" team showed the polymorphic selectionthat that occurred in the initial stages of structure formation, as well as specific blocks based on each spatial group.
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The mechanism of macrophage promoting the development of polymorphic glioblastoma is revealed.
Time of Update: 2020-08-09
On July 19th, the international academic journal Molecular Cell published the latest research results of the Yang Yanwei Research Group at the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences: Macrophage-associated PGK1 phosphorylation promotes saerobic glycolyis and tumorigenesis.
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Comparison of microsatellite polymorphism differences between transcription groups and genome screening.
Time of Update: 2020-08-06
Xia Yun, a member of the Chengdu Institute of Biological Research of the Chinese Academy of Sciences, discussed the effects of different sources, different splicing methods and sequencing depth on microsatellite screening results against the difficulty of interspecies proliferation of amphibious microsatellites, while high-throughput sequencing is relatively convenient.
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rs2273535 T-A polymorphism is associated with an increased risk of cancer.
Time of Update: 2020-07-30
!---- Recently, the Jinfu team of the Hefei Institute of Physical Sciences of the Chinese Academy of Sciences demonstrated that aurora kinase AURKA rs2273535 T-gt;A polymorphism was associated with an overall increase in cancer risk, especially breast cancer.